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. 2022 May 16;119(21):e2015576119. doi: 10.1073/pnas.2015576119

Fig. 2.

Fig. 2.

ascr#10 increases physiological cell death in the germline. (A) Schematic representation of one arm of hermaphrodite gonad showing approximate location of dying cells (green). (B) Representative SYTO12-stained gonads from hermaphrodites aged on or off ascr#10. (Scale bars, 10 μm.) (C) Numbers of dying cells, identified using two different methods, in gonads of adult hermaphrodites exposed to ascr#10 since early adulthood. (D) Increased germline cell death on ascr#10 requires CED-3. (E) ascr#10 increases incidence of cell death in mutants defective in several types of germline apoptosis. (F) Mutations that block cell death do not prevent increased GPC proliferation in the presence of ascr#10. (G) Blocking GPC proliferation prevents increased cell death on ascr#10. D through F show results from hermaphrodites on day 3 of adulthood, and G shows day 2 of adulthood. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. See Table S1 for primary data and details of statistical analyses.