. Author manuscript; available in PMC: 2023 Apr 19.

Published in final edited form as: Biochemistry. 2022 Apr 5;61(8):712–721. doi: 10.1021/acs.biochem.2c00085

Table 2.

Thermodynamic characteristics of carbamylation of ubiquitin amines with 80x and 120x NCO derived from the temperature series.^a

	10x sodium cyanate		15x sodium cyanate
Amine	ΔH^‡ kcal mol⁻¹	ΔS^‡ cal mol⁻¹ K⁻¹	ΔH^‡ kcal mol⁻¹	ΔS^‡ cal mol⁻¹ K⁻¹
K6	24 ± 10	−34 ± 33	17 ± 5	−56 ± 15
K48	22 ± 5	−38 ± 18	14 ± 2	−63 ± 7
K33	23 ± 6	−36 ± 20	19 ± 7	−50 ± 22

The enthalpies (ΔH^‡) and entropies (ΔS^‡) of activation were determined from linear regression fit of the temperature-dependent carbamylation reaction rates using Equation 1. The errors represent the standard errors of the linear regression.