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. 2022 Apr 6;7(6):1258–1267. doi: 10.1016/j.ekir.2022.03.030

Figure 1.

Figure 1

Autologous Tregs for generation of antigen-specific CAR-Tregs. Naive Tregs (CD4+/CD45RA+/CD25+/CD127low/neg) will be isolated from HLA-A∗02–negative renal transplant recipients designated to receive mismatched HLA-A∗02–positive organs (1). The isolated Tregs will be transduced with a lentiviral vector encoding the HLA-A∗02-CAR (2) and expanded ex vivo before cryopreservation (3). After renal transplantation, transplant recipients will receive an intravenous infusion of the autologous HLA-A∗02-CAR-Tregs (4). CAR, chimeric antigen receptor; CD, cluster of differentiation; HLA-A∗02, human leukocyte antigen class I molecule A∗02; Tregs, regulatory T cells.