Extended Data Fig. 5. PHP.GFAP-IL-2 expands regulatory T cells in the brain without impacting draining lymph nodes.
Mice were treated with PHP.GFAP-GFP or PHP.GFAP-IL-2 and assessed for Treg numbers by flow cytometry of perfused mice (n = 4-5, 4-9). a, Frequency of Treg cells, as a proportion of CD4 T cells in the superficial cervical lymph nodes (n = 4, 4), and b deep cervical lymph nodes (n = 4, 4). c, Absolute number of Treg cells in superficial cervical lymph nodes (n = 4, 4), and d deep cervical lymph nodes (n = 4, 4). e, Frequency and f absolute number of Treg cells in the pia mater, 14 days after PHP.GFAP-GFP or PHP.GFAP-IL-2 treatment (n = 5,5). g, Blood, spleen and perfused mouse brain from PHP.GFAP-GFP and PHP.GFAP-IL-2-treated mice were compared by high-dimensional flow cytometry for Treg numbers (n = 6, 6 blood; 12, 11 spleen and brain). h, Perfused organs from PHP.GFAP-GFP and PHP.GFAP-IL-2-treated mice were compared by flow cytometry for Treg frequency (n = 5/group). mLN, mesenteric lymph nodes; SC, spinal cord; IEL, intraepithelial leukocytes; LPL, lamina propria leukocytes; PP, Peyer’s Patch. Statistical analyses were performed using unpaired, non-parametric Mann–Whitney U-tests.