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. 2022 May 26;23(6):878–891. doi: 10.1038/s41590-022-01208-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — Astrocyte Ca²⁺ imaging in acute brain slices. a, Left, SR101⁺ astrocytes (dotted circles). Right, Fluo4 signal at baseline and following Phenylephrine (PHE) application. b, Averaged ΔF/F0 traces ± s.e.m. c, Boxplots of ΔF/F0 amplitude (left) and Area Under the Curve (AUC) (right). n = 3 mice/group and n_astrocytes = 710, 774 for control and αCamKII^IL-2. Whiskers represent maximal and minimal values. For AUC, Minimum (240.1, 249.1), 25% Percentile (338.1, 338.7), Median (381.8, 385.1), 75% Percentile (443.8, 442), Maximum (699.3, 702.3). For ΔF/F0 amplitude, Minimum (1.006, 1.007), 25% Percentile (1.604, 1.648), Median (2.139, 2.161), 75% Percentile (2.664, 2.665), Maximum (4.542, 4.239) d, Averaged ΔF/F0 traces ± s.e.m. e, Boxplots of the ΔF/F0 amplitude (left) and AUC (right). n = 3 mice/group, and n_astrocytes = 609, 646 for PHP.GFAP-GFP and PHP.GFAP-IL-2, respectively. Whiskers represent maximal and minimal values. For ΔF/F0 amplitude: Minimum (1.086, 1.075), 25% Percentile (1.578, 1.617), Median (2.007, 1.980), 75% Percentile (2.431, 2.348), Maximum (4.463, 3.489). For AUC: Minimum (280.6, 279.3), 25% Percentile (332.3, 339.7), Median (366.4, 371), 75% Percentile (408.7, 409.2), Maximum (704.4, 659.5). Neuronal function was measured using fEPSPs in PHP.GFAP-GFP and PHP.GFAP-IL-2 treated mice (n = 4,4). Input-output curves were recorded by applying single-stimuli. Slope f, and g, amplitude were analyzed. LTP was induced by applying three high frequency stimulus trains (100 stimuli; 100 Hz; arrows theta burst stimulation - TBS). After baseline, fEPSPs were measured. Changes in h, slope and i, amplitude were analyzed across time. Boxplots of the baseline (left) and final LTP (right). Mean ± s.e.m. Whiskers represent the maximal and minimal values. For slope at baseline: Minimum (0,08681, 0,09894), 25% Percentile (0.08875, 0.1164), Median (0.1248, 0.1473), 75% Percentile (0.3407, 0.2212), Maximum (0.4659, 0.2584); for slope of final LTP: Minimum (154.8, 169.4), 25% Percentile (159.4, 169.7), Median (184, 174.9), 75% Percentile (204.8, 214.1), Maximum (208.3, 225.8). For amplitude at baseline, Minimum (0.1941, 0), 25% Percentile (0.2298, 0.2979), Median (0.3279, 0.3897), 75% Percentile (0.8083, 0.5444), Maximum (1.139, 0.7234). For amplitude of final LTP: Minimum (166.9, 148.7), 25% Percentile (171, 155.3), Median (188.7,177.2), 75% Percentile (204.8, 181.9), Maximum (208.4, 1828). Unpaired, non-parametric Mann–Whitney U-test.

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