Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 2;25(6):688–701. doi: 10.1038/s41593-022-01084-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a. IHF labeling of 28-month-old APP51/TRGL layer V cortical neurons with LY marker (CTSB) and 3D6 monoclonal antibody against APP-βCTF/Aβ. Representative plane from a Z-stack (see also Extended Data Fig. 7a) of an early stage: 3D6 accumulates in CTSB positive perikaryal pa-AL of a normal looking cell (pa-AL, yellow arrow) and in those of a bleb-forming cell (bleb, white arrowhead). Scale bar 10 μm. b. IHF labeling of 30-month-old APP51 layer V cortical neurons with LY membrane marker (LAMP2), CTSB and 3D6. Representative single plane (top panel) and respective Z-stack series (1 mm-thick z1-z3, 2^nd to 4^th panel) of an intermediate stage: 3D6 accumulates in a LAMP2 and CTSB double-positive bleb originated from the perikaryal protrusion of a degenerating neuron (arrowhead and respective series), as opposed to a 3D6-negative neuron with normal perikaryal morphology (arrow and respective series). Scale bar 10 μm. c. IHF labeling of 28-month-old APP51/TRGL layer V cortical neurons with CTSB and 3D6. Representative IHF image of late stage: 3D6 co-localizes with CTSB in a bleb containing pa-AL of a mature PANTHOS neuron (arrowhead), maintaining a similar spatial segregation of 3D6 and CTSB immunoreactivity as seen in earlier stages (pa-AL). Filamentous/fibrillar 3D6 signal also emanates from the center of the PANTHOS. Scale bar 10 μm. d. IHF labeling of APP-βCTF/Aβ (4G8) in 5-month-old 5xFAD/TRGL mouse brain. Representative IHF image of a late stage PANTHOS neuron with intraneuronal Aβ occupying the central area with a faded/disappeared nuclear-DAPI fluorescence. Scale bar 10 μm. e. Representative amyloid (3D6) (that is, full view images for the one shown in Fig. 6f), amyloid (4G8), AV (LC3), and AL/LY (CTSD) IEM images of 5-month-old 5xFAD/TRGL mouse brain. Yellow arrowheads denote AVs and red arrows denote amyloid bundles. Scale bar 1 μm (3D6, CTSD) and 500 nm (LC3). a-e: Experiment was repeated 3 times independently with similar results.