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. 2022 Jun 2;25(6):688–701. doi: 10.1038/s41593-022-01084-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a. Representative fluorescence images of intraneuronal Aβ in autolysosomes (arrowhead, autophagy (LC3)/lysosomal (CTSD)) together with DAPI for nucleus. Scale bar 20 μm. b. Representative LC3/CTSD fluorescence images depicting a neuron with focal plasma membrane blebbing as pa-AL enlarge and proliferate (arrowhead). c. Z-stacked image series (1 μm thick, number z1–z5) showing LC3 and CTSD positive blebs emanating from perikaryon marked by DAPI staining. Scale bar 10 μm. d. Patterns of AV-related pathology showing a neuronal perikaryon with an intact nucleus. Enlarged LC3- and CTSD-positive vesicles (AL) are contained within numerous perikaryal membrane blebs Scale bar 10 μm. e. IHF labeling of Aβ (4G8) and DAPI stain. Perinuclear intraneuronal Aβ accumulation surrounding visible DAPI-positive nucleus within a PANTHOS like neuron. Scale bar 10 μm. a-d: Experiment was repeated 3 AD human brain independently with similar results.