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. 2022 May 27;23(6):892–903. doi: 10.1038/s41590-022-01220-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — (a) Immunoblot for Flag and tubulin loading control using TCL from NLRP11^Flag and NLRP11^ΔPYD-Flag cells sorted into low (lo) and high (hi) expressing populations. The gap in the blot indicates removal of additional NLRP11-expressing cell populations not included in this study. (b) Immunoblot for Flag, ASC, NLRP3, caspase-1 and tubulin loading control using TCL from NLRP11^KO, ASC^KD and NLRP3^KO cells restored with NLRP11-Flag. (c) ASC crosslinking and immunoblot for ASC using TCL and crosslinked TCL from Ctrl^KD, NLRP11^KD#1, NLRP11^KD#2, and NLRP3^KO cells left untreated, primed with LPS (200 ng ml⁻¹, 4 h) or primed+activated with Nig (5 μM, 20 min). Arrowheads mark crosslinked ASC polymers.

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