Extended Data Fig. 6. NLRP11 domain expression for NLRP3 oligomerization.
(a) Immunoblot for Myc, Flag and tubulin loading control using TCL from HEK293NLRP3-EGFP cells transiently cotransfected with empty vector (Ctrl), Myc or Flag-tagged NLRP11 or NLRP11 domains as indicated. (b) Immunoblot for Flag and tubulin loading control using TCL from NLRP11KO THP-1 stably restored with NLRP11-Flag or truncated NLRP11-Flag, as indicated. *marks a cross-reactive protein.