Extended Data Fig. 7. NLRP11 NACHT interactions and NLRP11 does not affect NEK7 recruitment to NLRP3.

(a,b) Immunoprecipitation with immobilized anti-Flag antibodies using TCL from HEK293 cells transiently transfected with Flag-NLRP11^NACHT, HA-NLRP3^NACHT and HA-NLRP12^NACHT, as indicated (a) using RIPA buffer and (b) washed with increasing NaCl concentrations (200 mM, 400 mM, 600 mM and 800 mM) and immunoblot of IP and TCL for HA and Flag. (c) IP with immobilized anti-NEK7 antibodies using TCL from untreated, Pam3CSK4 primed (1 μg ml⁻¹, 2 h) and primed+Nig (5 μM, 10 min) activated Cas9^Ctrl, NLRP11^KO and NLRP3^KO cells and immunoblot of IP and TCL for NLRP3, NEK7 and tubulin loading control.

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