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. 2021 Jun 4;29(6):825–838. doi: 10.5551/jat.62796

Supplementary Fig.2. Hepatic levels of reduced and oxidized glutathione and citrate synthase activity of LDL receptor knockout mice (LDLr−/−) treated with or without 2,4-dinitrophenol (DNP) .

Supplementary Fig.2. Hepatic levels of reduced and oxidized glutathione and citrate synthase activity of LDL receptor knockout mice (LDLr−/−) treated with or without 2,4-dinitrophenol (DNP)

(A) Hepatic reduced glutathione (GSH). (B) Hepatic oxidized glutathione (GSSG). (C) GSH/GSSG ratio (n=16). (D) Liver citrate synthase activity (n=9-10). Reduced (GSH) and oxidized (GSSG) glutathione levels were determined in liver homogenates as described by Teare et al (1993). Citrate synthase activity was determined by the conversion of oxaloacetate and acetyl-CoA to citrate and SH-CoA and monitored by the appearance of the thionitrobenzoic acid product at 412 nm.

Teare, JP, Punchard, NA, Powell, JJ, et al., Automated spectrophotometric method for determining oxidized and reduced glutathione in liver, Clinical chemistry, 1993; 39: 686-689.