Fig.3. Oxidant production in peritoneal macrophages and aorta and spontaneous atherosclerosis in male LDL receptor knockout mice (LDLr^−/−) treated with or without 2,4-dinitrophenol (DNP) .

(A) H₂O₂ release in peritoneal macrophages incubated with or without 100 nM phorbol 12-myristate 13-acetate (PMA) (n=5-9, ^＊p=0.025). (B) Global (DCF) oxidant production in aortic rings (n=7-8, ^＊p=0.025). (C) H₂O₂ release in aortic rings probed with Amplex red (n=8-11, ^＊p=0.036). (D) Lipid-stained areas of atherosclerotic lesions in the aortic root and representative images (n=14-17, ^＊p=0.033).