Fig.4. Peritoneal macrophage superoxide production and diet-induced atherosclerosis in LDL receptor knockout male and female mice (LDLr^−/−) treated with 2,4-dinitrophenol (DNP) and fed with an atherogenic diet .

(A) Superoxide production (DHE) in peritoneal macrophages incubated with or without 100 nM phorbol 12-myristate 13-acetate (PMA) (n=4-6, ^＊p=0.01). (B) Mitochondrial superoxide production (Mitosox) and representative images of peritoneal macrophages (n=5-6, ^＊p=0.03). (C) Lipid-stained areas of atherosclerotic lesions in the aortic root and representative images (n=9-15, ^＊p=0.026). (D) Anti-CD68 (macrophage marker) stained areas of lesions in the aortic root and representative images (n=7-8, ^＊p=0.024).