Figure 1.
SARS-CoV-2-specific antibody responses and B cell signatures detected in SARS-CoV-2 exposed children and adults
(A) Cohort of SARS-CoV-2 exposed children and adults.
(B) Number of participants who tested SARS-CoV-2 PCR positive, negative, or were undetermined or untested (ND).
(C) Age of SARS-CoV-2 exposed children (n = 53) and adults (n = 47). Data are shown as mean with SD.
(D) Number of children and adults with 2 or more symptoms between days −2 and +14 of PCR testing, no symptoms or were undetermined/untested (ND).
(E) Number of individuals with each HLA of interest (left) and number of simultaneous HLAs of interest (right).
(F) Distribution of days post PCR testing in children and adults (mean, SD).
(G) SARS-CoV-2 RBD- and N-specific IgG dilution curves with participants designated according to PCR and RBD IgG ELISA status (NA, not available).
(H) RBD- and N-specific IgG, IgM, and IgA antibody endpoint titers in children and adults (mean, SD).
(I) Staining profile of class-switched B cells (CD19+IgD−) with Spike probe in seroconverted (RBD IgG+ from ELISA) and non-seroconverted (RBD IgG−) individuals.
(J) Frequencies of Spike+ B cells as a proportion of CD19+IgD− B cells in PBMCs from RBD IgG+ or IgG− children and adults; data are shown as median with IQR. The statistical significance was determined with Kruskal-Wallis test.
(K) Isotype distribution of Spike+ B cells from RBD IgG+ or IgG− children and adults (mean, SD). The statistical significance was determined with Sidak’s multiple comparisons test.
(L) Correlation of Spike+ B cells against RBD IgG endpoint titers in SARS-CoV-2 exposed children and adults. The statistical significance was determined using Spearman’s rank correlation (rs).
See also Figure S1.