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. 2022 Feb 22;126(12):1824–1833. doi: 10.1038/s41416-022-01742-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic representation of the experimental setup. b Dose–response curves for five non-PM CRC-derived organoids and threePM-derived organoids. The dose range of oxaliplatin was 1, 100, 200, 300, 400 and 500 μM. Three days after a 1 h exposure to oxaliplatin the fraction of remaining viable cells was measured using Cell Titer Glo and plotted as percentage of untreated control cells. c Boxplot showing area under the curve (AUC) values, calculated from the dose–response curves. d The contribution of cancer-associated fibroblasts (CAFs) and N-acetyl-cysteine (NAC) in glutathione synthesis. GCLC, the rate-limiting enzyme in this pathway, is indicated in red. e GCLC mRNA expression levels in non-PM and PM-derived organoids, measured by RNA sequencing. f, g GCLC protein expression levels in non-PM and PM-derived organoids in relation to actin. The boxplot in (f) shows optical density measurements of the exposed films in (g), normalised to actin.