Table 2.
HPLC based phenolic fingerprinting and quantification of the major polyphenols in ethyl acetate fraction of crude methanol extract of Acacia nilotica.
| Compound | RT (min) | Quantification (μg/mg) | Molecular formula | Molecular mass |
|---|---|---|---|---|
| Gallic acid | 3.57 | 156.26 | C7H6O5 | 170.12 |
| Catechin | 4.83 | 265.19 | C15H14O | 290.27 |
| Epicatechin | 5.61 | 196.35 | C15H14O | 290.27 |
| Rutin | 7.10 | 109.78 | C27H30O16 | 610.52 |
| Umbelliferone | 9.24 | 183.17 | C9H6O3 | 162.14 |
| o-Coumaric | 10.68 | t | C9H8O3 | 164.16 |
| Quercetin | 12.95 | 161.91 | C15H10O7 | 302.24 |
| Myricetin | 11.09 | 117.29 | C15H10O8 | 318.24 |
| Betulin | 15.42 | 56.83 | C30H50O2 | 442.72 |
| Kaempferol | 16.10 | 19.27 | C15H10O6 | 286.24 |
RT: Retention Time.
“t” indicates “trace”.