Skip to main content
. 2022 Jun 7;8:277. doi: 10.1038/s41420-022-01077-3

Fig. 1. mTORC1, but not mTORC2, is indispensable for adipogenesis and vice versa.

Fig. 1

Murine MSCs (C3H10T1/2) were subjected to osteogenic differentiation, and lysates were collected at the indicated time points and subjected to A Western blot analysis using the indicated antibodies and B RT-PCR. C C2C12 cells (skeletal myocytes) were subjected to osteogenic transdifferentiation, and cell lysates were subjected to Western blotting and D RT-PCR. Murine MSCs were subjected to adipogenic differentiation, and cell lysates were collected at the indicated time points and E subjected to Western blotting and F RT-PCR as indicated. U2OS cells were subjected to adipogenic transdifferentiation, and cell lysates were collected and G subjected to Western blot analysis and H RT-PCR analysis as indicated. Mean ± S.E.M.; N = 3, *p < 0.1 versus control, **p < 0.01 versus control; ***p < 0.001 versus control. RUNX2 runt-related transcription factor 2, OCN Osteocalcin, Adipo Q adiponectin Q, PPAR-γ peroxisome proliferator-activated receptor gamma.