FIG. 7.

Membrane permeabilization induced by plant defensins and α-PT in S. cerevisiae wild-type cells and S. cerevisiae DM1 cells in low- and high-ionic-strength growth media. Dose-response curves of membrane permeabilization measured by SYTOX Green fluorescence and growth inhibition of S. cerevisiae wild-type cells and S. cerevisiae DM1 cells suspended in YMM (A) and YMM plus 5 mM MgCl₂ (B) are shown. Fungal cells were incubated with Rs-AFP2, Hs-AFP1, or Dm-AMP1 for 12 h, whereupon fluorescence was measured. Values correspond to one representative experiment of two.