Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 5;65(7):1185–1197. doi: 10.1007/s00125-022-05708-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Changes of immune cell profiles of the islets. Representative immunostaining images of the pancreases of mice in the three groups (a–c). (a) Pancreas from a mouse not receiving anti-PD-L1 monoclonal antibody (mAb) or hMSCs (control: normoglycaemic, insulin content was 2.17 pmol/mg). (b) Pancreas from an MSC(−) mouse (normoglycaemic, insulin content was 0.41 pmol/mg). (c) Pancreas from an MSC(+) mouse (normoglycaemic, insulin content was 1.00 pmol/mg). (a–c) Upper panels: H&E staining. Middle panels: quadruple immunofluorescence staining for DAPI (light blue), insulin (red), CD3 (blue) and Mac-2 antigen (green), and their merge. Lower panels: quadruple immunofluorescence staining for DAPI, insulin, CD3 and CXCL9 (green), and their merge. The upper to lower panels are 2 μm serial sections. Scale bars, 200 μm. (d–f) Comparison of the immunostaining-positive area of the pancreas between groups. (d) CD3-positive area. (e) Mac-2 antigen-positive area. (f) CXCL9-positive area [control group islets: n=15; MSC(−) group islets: n=15; MSC(+) group islets: n=15]. Data are shown as the mean ± SEM. *p˂0.05; ***p˂0.001 between the indicated two groups by Student’s t test