Figure 2.

LVS infection upregulates PMN glycolysis. Glycolytic function measured by Seahorse analysis as extracellular acidification rate (ECAR) in control and LVS-infected PMNs, n=3. (A) ECAR curves at 12 hr. Glucose was injected to a final concentration of 10 mM at 20.84 min, oligomycin was injected to a final concentration of 1 µM at 46.78 min and 2-DG was injected to a final concentration of 50 mM at 72.83 min. Where not visible, error bars are smaller than symbols. Quantitation of glycolysis (B–D) and glycolytic capacity (E–G) in control and LVS-infected PMNs at 8, 12 and 24 hpi. *p < 0.05. **p < 0.01. ns, not significant.