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Glycolysis inhibition blocks the ability of LVS to delay PMN apoptosis. (A) Effects of 2-DG on the kinetics of apoptosis of control and LVS-infected cells were measured using Annexin V-FITC staining and flow cytometry at the indicated time points, n=4. ***p < 0.001, ****p < 0.0001, ns, not significant, as indicated. (B) Effects of 3PO on PMN viability at 24 hr as measured by PI staining and flow cytometry, n=3. *p<0.05, ns, not significant, as indicated.
