Figure 9.

Caspase inhibition increases PMN glycolysis, glycolytic capacity, and glycogen abundance. (A) Kinetics of apoptosis of control and Q-VD-OPh-treated PMNs were measured using Annexin V-FITC staining and flow cytometry. Data are the mean ± SEM, n=3. ****p < 0.0001 compared to untreated, uninfected PMNs, ^‡ p < 0.05 and ^‡‡‡‡ p < 0.0001 compared to untreated, LVS-infected PMNs at the indicated time points. (B) Quantitation of glycolysis and glycolytic capacity measured by Seahorse analysis as extracellular acidification rate (ECAR) in PMNs in the presence or absence of Q-VD-OPh at 12 hr, n=4. ***p < 0.001. (C) Measurement of glycogen abundance in PMNs in the presence or absence of Q-VD-OPh at 24 hr, n=6-7. **p < 0.01.