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. 2022 Jun 7;13(6):533. doi: 10.1038/s41419-022-04979-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A H&E staining of paraffin-embedded prostatic tissue specimens from the three groups of patients. Scale bar: 100 μm. B Computer image analysis for the ratio of stromal-to-epithelial hyperplasia in the three groups. The variance was similar between the groups. ***P < 0.001. C Masson’s trichrome staining for prostatic tissue specimens from the patients of three groups. Scale bar: 100 μm. Right bar graph is the IOD of collagen fiber with blue staining. The variance was similar between the groups. **P < 0.01, ***P < 0.001. D, E Computer image analysis of Masson’s trichrome staining and the ratio of the stromal-to-epithelial area. The proportion of collagen fiber in the total prostatic adenoma (D) and in the stromal area (E) of the three groups. The variance was similar between the groups. **P < 0.01, ***P < 0.001. F Prostatic tissues from the three groups of patients were stained for α-SMA (red) and collagen I (green), and the nuclei were counterstained with DAPI (blue) by immunofluorescence. Merged images indicate a myofibroblastic phenotype. Scale bar: 50 μm.