Skip to main content
. 2022 Jun 7;13(6):533. doi: 10.1038/s41419-022-04979-3

Fig. 3. Estrogen promotes prostatic fibrosis in early-progressed BPH patients by activating GPER/Gαi signaling.

Fig. 3

A IHC staining for ERα, ERβ, and GPER in prostatic tissue specimens from the three groups. Scale bar: 100 μm. B, C Prsc (B) and WPMY-1 (C) were treated with G1 at 0 nM (DMSO), 1 nM, 10 nM, and 100 nM for 48 h. Q-PCR analysis was used to detect the expression of TGF-β1, α-SMA, and COL1A1. The variance was similar between the groups. *P < 0.05. D, E Prsc (D) and WPMY-1 (E) were treated with or without 1 nM E2 in the presence or absence of 1 µM G15 for 48 h, and TGF-β1, α-SMA, COL1A1, and Lox were analyzed by Q-PCR. The variance was similar between the groups. *P < 0.05. F, G WPMY-1 and Prsc were treated with 1 nM E2 plus 1 µM G15 (F) or 1 nM G1 plus shGPER (G) for 72 h, and collagen I, α-SMA, and Lox were detected by western blot. H The knockdown efficiency of shGPER in WPMY-1 and Prsc. I WPMY-1 were treated with 1 nM E2 or 1 nM G1 plus PTX at 0.1 µg/ml for 72 h, and collagen I, α-SMA, and Lox were detected by western blot. J WPMY-1 were treated with 1 nM G1 and were transduced with or without the three subunits of Gαi-shRNA for 72 h, and collagen I, α-SMA, Lox, and Gαi were detected by western blot. GAPDH was used as a loading control.