TABLE 2.
Distribution of microorganisms in a pozol ball as estimated by plate enumeration or most-probable-number counting with different culture media
Mediuma | Organisms targeted | No. of cells (log CFU · g [dry wt]−1) at theb:
|
||
---|---|---|---|---|
Periphery | Intermediate position | Center | ||
PCA (all colonies) | Total flora | 9.79 (8.78) | 9.15 (8.02) | 9.14 (8.31) |
PCA (large colonies) | Non-LAB | 8.47 (7.18) | 5.73 (4.78) | 5.72 (4.75) |
MFT | Total flora | 8.87 (8.09) | 7.89 (7.24) | 7.87 (7.15) |
MRS-glucose | LAB | 8.82 (7.75) | 8.99 (7.72) | 8.89 (7.49) |
M17 | Streptococcus, Lactococcus | 8.63 (7.77) | 7.78 (7.01) | 8.32 (7.32) |
MRS-starch | Amylolytic LAB | 8.69 (8.28) | 8.71 (8.18) | 8.72 (8.18) |
LEU | Leuconostoc | 8.81 (8.05) | 6.45 (6.02) | 5.12 (5.18) |
VRBGc | Enterobacteria | 7.80 (7.28) | 5.91 (5.01) | 3.88 (3.85) |
ENC | Enterococcus | <4 | <4 | <4 |
LFB | Lactate-fermenting strict anaerobes | <4 | <4 | <4 |
PDA | Yeasts | 7.82 (7.14) | 6.08 (5.24) | <4 |
PDA | Fungi | 6.85 (6.78) | 5.34 (5.00) | <4 |
For an explanation of the media see the text.
The data are means based on three replicates for a single pozol ball. The values in parentheses are standard deviations.
VRBG, violet red bile agar.