TABLE 4.

Percentages of the total SSU rRNA in pozol samples as determined with individual probes

Probe(s)	Target taxon(a)	% of total SSU rRNA at thea:
		Periphery	Intermediate position	Center
Univ1390	Universal	100	100	100
Eub338	Eubacteria	88.9	75.4	98.2
Euc502	Eucarya	6.09	4.65	2.25
Eub338 + Euc502	Control for universal probe	95.0	80.1	100.5
Entero	Enterobacteria	0.85	NDb	ND
Lacb0722	LAB	88.6	97.4	94.3
Strc493	Streptococcus, Lactococcus, some Leuconostoc spp.	26.9	26.8	54.2
Lab158	Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, Weisella	68.1	65.20	59.5
LU2	Leuconostoc	6.82	3.84	4.08
212RLa	Lactococcus	0.27	0.74	1.47
Lab158 + Strc493 − LU2	Control for all LAB probes	89.2	88.2	99.6

^aThe data are expressed as percentages of the signal obtained with universal probe Univ1390 and are means based on three replicates. The amounts of total RNA recovered at the periphery, the intermediate position, and the center were 29, 17.7, and 13.3 μg/g of pozol, respectively. 

^bND, not detected.