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. 2022 May 25;13:875788. doi: 10.3389/fimmu.2022.875788

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Copyright © 2022 Sapra, Shokeen, Porwal, Saini, Bhardwaj, Mathew, Mishra, Chattopadhyay, Dar, Verma and Srivastava

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BL administration attenuates bone loss in Ovx mice: (A) Experimental layout followed for in vivo studies. Mice were divided into 3 groups, viz., sham, Ovx, and Ovx + BL groups, that received BL at 10⁹ CFU/day orally reconstituted in drinking water. At the end of 45 days, mice were sacrificed and analyzed for various parameters. (B) 2D SEM images. (C) 2D MATLAB analysis of SEM images. (D) 3D AFM images. (E) 3D MATLAB analysis of AFM images. The representative images are indicative of one independent experiment, and comparable results were obtained in two different independent experiments with n =6 mice/group/experiment.