Figure 6.

Immune training with β–glucan alters monocyte phenotype. Isolated human monocytes were trained with β–glucan or vehicle control and allowed to rest for 7 days. After 7 days, monocytes were treated with LPS (10ng/mL) or PBS (baseline control) for 24 hours. Cell surface markers were analyzed by flow cytometry. (A) Scatterplot depicting the percent of isolated CD14+ monocytes expressing CD16 in LPS–stimulated and unstimulated cells. (B) Mean fluorescence of CD11b/Mac–1. (C) Mean fluorescence of cell surface CD123. (D) Mean fluorescence of cell surface HLA–DR. (E) Mean fluorescence of cell surface CD40. All results analyzed using a mixed effect model and Šídák’s multiple comparisons test (*P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001) (N=10–14). ns, not significant.