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. 2022 May 25;12:873516. doi: 10.3389/fonc.2022.873516

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Copyright © 2022 Nunes-Xavier, Mingo, Emaldi, Flem-Karlsen, Mælandsmo, Fodstad, Llarena, López and Pulido

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and subcellular localization of PDK2 and PDK3 in PCa cells. (A) Immunoblot of ectopically expressed PDK2-Flag, PDK3-Flag, and Flag-PTEN (as a control) in COS-7 and LNCaP cells using anti-Flag antibody. (B) Immunofluorescence of PDK2-Flag, PDK3-Flag, and Flag-PTEN in COS-7 cells, using anti-Flag antibody (green). (C) Immunofluorescence of PDK2-Flag and PDK3-Flag (green) as in B, with Mitotracker as a mitochondria marker (red). (D) Immunofluorescence of PDK2-Flag and PDK3-Flag (green) in LNCaP cells, with Mitotracker as a mitochondria marker (red). In (B–D) nuclei were stained with DAPI (blue). Note the punctuated and mitochondrial localization of PDK2 and PDK3, as compared to the cytoplasmic PTEN localization.