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. Author manuscript; available in PMC: 2022 Jun 8.
Published in final edited form as: J Vis Exp. 2022 May 18;(183):10.3791/63189. doi: 10.3791/63189

Figure 4: Brain culture and immunostaining.

Figure 4:

(A) Whole brains in a 12-well culture dish containing 1 mL of SSM. The culture dish is then placed in a 25 °C incubator for 24 h. (B) 72-well mini tray that holds brain explants during immunostaining. Brains are washed and solutions transferred using a P20 micropipette set to 10 μL.