Table 2: Recipe for making supplemented Schneider’s media (SSM).

The table lists the volume of all ingredients required for preparing 5 mL of SSM.

Ingredient (starting concentration)	To make 5 mL	Final concentration
Schneider’s Drosophila media	3.89 mL
Fetal bovine Serum (100%)	500 μL	10%
Glutamine (200 mM)	500 μL	20 mM
Penicillin (5000 units/mL), Streptomycin (50,000 μg/mL)	100 μL	1000 U/mL Pen, 1 mg/mL Strep
Insulin (10 mg/mL)	10 μL	0.02 mg/mL
glutathione (50 mg/mL)	5 μL	0.05 mg/mL
In a sterile hood, using sterile technique, add all ingredients together in a 14 mL conical tube. Place it on ice until use.