Table 3: Recipe for making fixative and PEM buffer.

The table lists the chemicals and their respective concentrations for preparing fixative and PEM buffer.

Recipe for making fixative
Ingredient (starting concentration)	To make 40 mL	Final concentration
EM grade formaldehyde 16%	10 mL	4%
PEM buffer pH 7.0	29.96 mL
Triton X-100	40 mL	0.10%
Mix all ingredients together. Aliquot 1 mL into microcentrifuge tubes and store at −20 °C. Do not refreeze the aliquots after thawing.
Recipe for PEM buffer
Ingredient (starting concentration)	To make 100 mL	Final concentration
PIPES pH 6.8 (500 mM)	20 mL	100 mM
EGTA (500 mM)	2 mL	10 mM
MgSO4 (1 M)	100 mL	1 mM
Water	77.9 mL