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. 2022 May 25;13:897487. doi: 10.3389/fimmu.2022.897487

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Copyright © 2022 Chen, Wu, Zhu, Chen, Xu, Tang, Jiao and Yu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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METTL3 regulates vascular endothelial barrier function in vitro. (A, B) TEER was used to measure endothelial permeability in HULEC-5a cells transfected with scramble or METTL3 shRNA (A) and in HULEC-5a cells with or without METTL3 overexpression (B). (C, D) Immunoblot showing the protein expression of VE-Cadherin and ICAM-1 in HULEC-5a cells transfected with scramble or METTL3 shRNA (C) and in HULEC-5a cells with or without METTL3 overexpression (D). (E, F) RT-qPCR analysis showing inflammatory cytokine mRNA expression in HULEC-5a cells transfected with scramble or METTL3 shRNA (E) and in HULEC-5a cells with or without METTL3 overexpression (F). All data are expressed as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.