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. 2022 May 25;13:886149. doi: 10.3389/fphys.2022.886149

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Copyright © 2022 Pietrangelo, Demontis, Santangelo, Pini, Bonelli, Rosato, Roberti, Locatelli, Tartaglia, Marramiero, Verratti, Bondi, Fulle, D’Aloja and D’Ovidio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results on undifferentiated thyrohyoid and ileopsoas hMPCs. (A) Population doubling level (PDL) of postmortem hMPCs derived from thyrohyoid muscle obtained from 40– (orange squared symbol) and from iliopsoas muscle obtained from 71– (green triangle symbols) year-old corpses while black symbols represent the PDL of five different hMPC populations derived from vastus lateralis of alive donors in the range of 40–71 years old. (B) Picture of thyrohyoid hMPCs in Petri dishes. (C) The graph shows mean and standard deviation of [Ca²⁺]_i measurement on undifferentiated thyrohyoid (UNDIF THY) and ileopsoas (UNDIF IL) hMPCs. They significantly differ with p ≤ 0.05. (D) Representative [Ca²⁺]_i oscillation recorded on thyrohyoid hMPCs. The bar in panel B represents 100 μm.