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. 2022 May 21;13(8):2694–2704. doi: 10.7150/jca.65822

Figure 5.

Figure 5

TMEM158 triggers EMT by activating the canonical and non-canonical TGF-β signaling pathways. (A) Western blot analysis of the TGF-β signaling pathway, phosphorylated ERK1/2, total ERK1/2 and epithelial/mesenchymal protein marker, in BT549 and HCC1187 cells. (B) Effect of the ERK phosphorylation inhibitor PD98059 on ERK signaling in TMEM158 overexpressing MDA-MB-231 cells. Western blotting analysis of proteins in the Smad-dependent pathway, phosphorylated ERK1/2, total ERK1/2 and epithelial/mesenchymal protein markers; the bar graph shows protein quantification. (C)-(E) The bar graphs show protein quantification. NC: negative control siRNA; Si1: TMEM158 siRNA1; Si2: TMEM158 siRNA2; OE: TMEM158 overexpression. *p<0.05, **p<0.01, ***p<0.001, ns: nonsignificant