Figure EV1. Binding activity and species specificity of ASC VHHs.
Lysates of HEK 293T cells transiently expressing HA‐tagged VHHASC (wild‐type or point mutants), an unrelated control (VHHNP1) and the indicated bait proteins fused to Renilla luciferase were used to immunoprecipitate VHHs with immobilized anti‐HA antibody. Renilla luciferase activity of the co‐immunoprecipitated proteins was measured and normalized to the input luciferase. Data represents mean values ± SEM from three independent experiments. ****P < 0.0001, One‐way ANOVA, multiple comparison (Tukey test). 'TYD‐KKK' indicates the triple mutant T57K, Y59K, and D62K.