Figure 2. VHH_ASC disrupts the “prionoid” activities and disassembles ASC specks.

1. Time‐lapse confocal imaging of the in vitro nucleation of soluble ASC‐mTurquoise (red) by ASC‐TagGFP (ASC‐GFP) specks (green), that were left untreated (None), or pre‐incubated with VHH_ASC or mutVHH_ASC (200 µg ml⁻¹ for 15 min). Scale bars: 10 μm.
2. Median fluorescence intensity (MFI) of mTurquoise graphed over time showing its polymerization seeded by ASC‐TagGFP specks. Each line shows the mTurquoise MFI around a seeding ASC‐TagGFP speck. Data is one representative of three independent experiments.
3. WES capillary electrophoresis and immunoblotting of DSS cross‐linked in‐vitro‐generated human ASC‐mTurquoise (ASC) specks that were incubated for 1 h (RT) with 2, 20 or 200 µg ml⁻¹ of VHH_ASC, mutVHH_ASC or with 20 µg ml⁻¹ of a polyclonal anti‐ASC Ab (a‐ASC pAb). Data is from one representative of four independent experiments.

Source data are available online for this figure.