Table 3.
Effect of inactivation protocols in salivary biomarkers of metabolic status, kidney function and tissue damage [median (25–75 percentile)].
| Analyte | TT1 | TT2 | TT3 | TT4 | TT5 | TT6 | P |
|---|---|---|---|---|---|---|---|
| AST, IU/L | 19.00 (16.5–20.95) | 7.9* (4.65–10.08) | 0.35* (0.15–0.85) | 0.75* (0.15–1.7) | 18.65 (13.3–20.95) | 19.45 (14.1–20.625) | < 0.001 |
| ALT, IU/L | 5.3 (3.825–7) | 1.65* (1.075–2.25) | 1.6* (1.375–2.075) | 1.55* (1.2–2.2) | 4.45 (2.925–6.1) | 4.05 (2.95–6.2) | < 0.001 |
| CK, IU/L | 19.75 (12.375–29.5) | 0.25* (0–2.075) | 0.3* (0–1.05) | 0.7* (0–3) | 15.95 (12.325–20.675) | 17.6 (12.175–21.5) | < 0.001 |
| LDH, IU/L | 293.3 (220.625–420.175) | 4.45* (2.75–5.8) | 1.8* (0.525–3.375) | 1.5* (0.45–4.05) | 283.6 (214.2–406.325) | 282.95 (216.375–417.8) | < 0.001 |
| Urea, mmol/L | 9.98 (8.60–10.75) | 10.0 (8.59–11.02) | 10.3 (8.76–11.21) | 9.10* (7.80–9.85) | 9.69 (8.31–10.77) | 9.73 (8.59–11.08) | < 0.001 |
| Creatinine, µmol/L | 7.96 (5.08–11.05) | 7.96 (5.08–11.27) | 6.19 (1.77–11.71) | 7.51 (5.75–10.17) | 7.51 (5.30–11.27) | 7.51 (6.19–9.50) | 0.382 |
| Total cholesterol, mmol/L | 6.1 × 10–3 (9.8 × 10–4–8.5 × 10–3) | 1.19 × 10–2 (5.18 × 10–3–1.67 × 10–2) | 1.26 × 10–2 (1.09 × 10–2–1.42 × 10–2) | 8.29 × 10–3 (1.17 × 10-3–1.84 × 10–2) | 8.03 × 10–3 (0–1.02 × 10–2) | 1.18 × 10–2 (2.85 × 10–3–1.37 × 10–2) | 0.0417 |
| Triglycerides, mmol/L | 0.031 (0.027–0.055) | 0.036 (0.025–0.057) | 0.034 (0.017–0.062) | 0.154* (0.120–0.188) | 0.105* (0.091–0.126) | 0.104* (0.093–0.117) | < 0.001 |
| Glucose, mmol/L | 0.014 (4.44 × 10–3–5,6 × 10–2) | 8.32 × 10–3 (3.89 × 10–3–6.32 × 10–2) | 3.33 × 10–3 (5,56 × 10–4–5,49 × 10–2) | 2.00 × 10-2* (5.56 × 10–4–9.88 × 10–2) | 2.83 × 10–2* (7.22 × 10–3–0.110) | 2.44 × 10–2* (7.22 × 10–3–0.109) | < 0.001 |
| CA, mmol/L | 1.28 (1.05–1.39) | 1.05 (0.85–1.29) | 1.04 (0.90–1.29) | 1.21 (1.03–1.44) | 1.24 (1.03–1.38) | 1.24 (1.03–1.42) | 0.114 |
| P, mmol/L | 4.70 (4.08–5.49) | 4.72 (4.27–5.42) | 4.74 (4.23–5.56) | 4.51* (3.79–5.25) | 4.49 (4.17–5.37) | 4.59 (4.17–5.49) | 0.001 |
| Lactate, µmol/L | 1.755 (0.905–2.33) | 1.805 (0.985–2.3275) | 1.8 (0.94–2.295) | 1.765 (0.3425–2.3125) | 1.705* (0.8175–2.113) | 1.745* (0.855–2.178) | < 0.001 |
| ChE, IU/L | 4.1 (2.3–8.45) | 3.1 (1.3–5.4) | 2.3* (0.8–3.6) | 2.8* (1.6–5.2) | 3.9 (1.3–6.0) | 4.2 (1.4–5.8) | < 0.001 |
Significant values are in bold.
*P < 0.05. Control (TT1); 65 °C 30 min (TT2); 92 °C 15 min (TT3); SDS (TT4); NP40 (TT5) and triton (TT6).