Figure 11.

ILK KD reduced IFNγ-induced PD-L1 expression in the CRC cell lines in part via regulating NF-κB p65. [(A), upper] western blot showing NF-κB p65 (total and phosphorylated) protein expression in CRC cell lines stimulated with IFNγ (50 ng) for 24 hr. [(A), lower] the quantitation of NF-κB p65 (total and phosphorylated) protein expression. Quantitation of proteins expression normalized to -Dox. Actin was used as an internal control. Error bars are represented as mean ± SEM (n = 3). P-value was analyzed with an unpaired t-test. The significant P-value is < 0.05. [(B), left] The correlation between ILK and CD274 gene expression in COAD tumors via GEPIA. [(B), right] The correlation between ILK and Rela gene expression in COAD tumors via GEPIA. R is Spearman’s Rho values. Significant P-value is < 0.05.