Sequences and thermal melting data for the PS/2′-OMe ONs used in assay. Melting temperatures against complementary RNA.
| ON | ON sequence (5′ to 3′) | T m °C |
|---|---|---|
| 20 |
|
72.8 |
| 21 |
|
60.5 |
| 22 |
|
68.1 |
| 23 |
|
66.1 |
| 24 |
|
70.7 |
| ON | ON sequence (5′ to 3′) | T m °C |
|---|---|---|
| 20 |
|
72.8 |
| 21 |
|
60.5 |
| 22 |
|
68.1 |
| 23 |
|
66.1 |
| 24 |
|
70.7 |