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| PNA sequencea (N–H/C–LysNH2) | Modification | T m DNA (°C) | T m RNA (°C) | T m mmRNA (°C) |
|---|---|---|---|---|
| CATTGTCACACT | None (aegPNA) | 65 | 66 | 55 |
| CATTGtCACACT | Ethano- (R = H) | 50 | 59 | 45 |
| CATTGtCACACT | Am-ethano- (R = H2NCH2–) | 52 | 60 | 47 |
| CATTGtCACACT | Gu-ethano (R = guanidinomethyl–) | 54 | 63 | 47 |
The modification site is denoted by a small letter.
