T m data of α-gem-dimethylated PNA with DNA and RNA27.
| |||
|---|---|---|---|
| Sequencea (N–H/C–NH2) | Modification | T m DNAb (°C) | T m RNAb (°C) |
| TTTTTTT-Lys | None (aegPNA) | 43 | 54 |
| TtTtTtTt-Lys | aedmgPNA | 80 | 73 |
| ttttttttt-Lys | aedmgPNA | >85 | >85 |
| TtTtTtTt-Lys | apdmgPNA | 59 | 57 |
| tttttttt-Lys | apdmgPNA | 77 | 61 |
| GTAGATCACT | None (aegPNA) | 50 (38) | 50 (40) |
| GtAGAtCACt | aedmgPNA | 81 (56) | 72 (32) |
| GtAGAtCACt | apdmgPNA | 73 (50) | 65 (33) |
a
The modification site is denoted by a small letter.
b
Antiparallel duplexes; Tm values for parallel duplexes are shown in parentheses.