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| |||
|---|---|---|---|
| Sequence (N–H/C–NH2)a | Substituent (R) | T m DNA (°C) | ΔTmb (°C) |
| GTAGATCACT-LLys | None | 51.4 | — |
| GtAGAtCACt-LLys | β-(S)-Me | 51.0 | −0.4 |
| GtAGAtCACt-DLys | β-(R)-Me | No melting | Large |
The modification site is denoted by a small letter.
ΔTm refers to Tm difference between the modified PNA and aegPNA.
