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. Author manuscript; available in PMC: 2022 Jun 8.
Published in final edited form as: Cell Rep. 2022 May 17;39(7):110824. doi: 10.1016/j.celrep.2022.110824

Figure 1. Generation and initial characterization of Tbc1d7 knockout mice.

Figure 1.

(A and B) Scheme for CRISPR-Cas9 targeting of Tbc1d7, with the identified 35-bp out-of-frame deletion (A) denoted and (B) detected by PCR genotyping.

(C and D) Immunoblots of tissue lysates from littermate Tbc1d7+/+ and Tbc1d7−/− mice at 6 weeks of age following a 6 h daytime fast. Phospho-S6 to total S6 ratios are quantified in (D) as mean ± SD. n = 3.

(E) Genotypes of 368 offspring of crosses between Tbc1d7+/− mice.

(F) Body weights from a cohort of littermate offspring from Tbc1d7+/− crosses over 13 months graphed as mean ± SD at each age. n = 13 Tbc1d7+/+, 19 Tbc1d7+/−, and 12 Tbc1d7−/−. Age 0 is the weight at weaning (21 days).

(G) DEXA scan analysis of the mice in (F) at 6 months of age, with the lean-to-fat-mass ratio graphed as mean ± SD.

Statistical analysis by one-way ANOVA, *p < 0.05.