TABLE 2.
Yield and activities of LCCI and LCCIa produced by P. pastoris SMD1168
| Enzyme and stepa | [Protein] (mg ml−1) | Total protein (mg) | Total activity (U)b | Sp act (mU mg−1) |
|---|---|---|---|---|
| LCCI | ||||
| Culture (200 ml) | 1.6 | 320 | 7.82 | 24 |
| Dialysis (10 ml) | 4.5 | 45 | 7.2 | 160 |
| Post-HPLC (50 ml) dialyzed to 7 ml | 0.8 | 5.6 | 3.68 (ABTS)c, 6.83 (DEPDA)c | 656 (ABTS)c, 1,220 (DEPDA)c |
| LCCIa | ||||
| Culture (200 ml) | 1.2 | 240 | 1.64 | 7 |
| Dialysis (10 ml) | 3.1 | 31 | 1.52 | 49 |
| Post-HPLC (50 ml) dialyzed to 5 ml | 0.5 | 2.5 | 0.54 | Inactive (ABTS)c, 216 (DEPDA)c |
a
HPLC, high-pressure liquid chromatography.
b
A unit of activity (U) is defined as the amount of enzyme, in milligrams, that will oxidize 10−6 mol of substrate per min with the corresponding reduction of dioxygen to water.
c
Substance in parentheses is the substrate used.