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. 2022 May 13;26(1):220. doi: 10.3892/mmr.2022.12736

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Copyright: © Lou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — CXCR4 overexpression reduces the alleviative effect of tranilast on LPS-induced apoptosis. (A) TUNEL analysis of apoptosis in the LPS-stimulated BEAS-2B cells, pretreated with tranilast (200 µM) and transfected with ov-NC or ov-CXCR4. (B) Western blot analysis of the apoptosis-related proteins, Bcl2, Bax and cleaved caspase-3 in LPS-stimulated BEAS-2B cells, pretreated with tranilast (200 µM) and transfected with ov-NC or ov-CXCR4. ***P<0.001 vs. control; ^##P<0.01, ^###P<0.001 vs. LPS; ^∆∆∆P<0.001 vs. ov-NC. LPS, lipopolysaccharide; ov, overexpression; NC, negative control; CXCR4, C-X-C motif chemokine receptor 4.