Figure 2.

Binding curves of fluorescent ligand binding to M₄ receptor. 1 nM (squares) or 6 nM (circles) UR-MK342 (a) or 2 nM (squares) or 20 nM (circles) UR-CG072 (b) incubated with different concentrations M₄ receptor. Total binding (filled symbols) was determined in the absence and non-specific binding (open symbols) in the presence of 1000-fold excess of UNSW-MK259 in the case of UR-CG072 or scopolamine in the case of UR-MK342. The 1 h measurement point for UR-CG072 and 5 h measurement point for UR-MK342 are shown and was used for calculations. The concentration of M₄ receptor binding sites (C_{M4 receptor}) was calculated post hoc from the results of these experiments using the model described in [27]. A representative experiment of at least three independent experiments is shown. Experiments were performed in duplicates with all measurement data points shown.