Figure 3.

Time course of FA change caused by UR-MK342 (a) or UR-CG072 (b) binding to M₄ receptors on the BBV particles. The reaction was initiated by the addition of 20 µl M₄ receptor displaying BBV particles to 6 nM UR-MK342 (a) or 5 nM UR-CG072 (b) in the absence (open blue square, filed blue circle) or presence (open black circle) of 6 µM (a) or 3 µM (b) scopolamine, respectively. After 180 min (indicated with an arrow) dissociation was initiated by the addition of 6 µM (a) or 3 µM (b) scopolamine (open blue square). An equivalent volume of assay buffer was added to association controls (filed blue circle). Representative experiments of at least three independent experiments are shown. ΔFA is calculated by subtracting the FA value of non-specific binding from the measured FA value of the corresponding measurement.