Figure 8.
Saturation binding of UR-CG072 binding to live CHO-K1-hM4R cells. The CHO-K1-hM4R cells (25000 cells per well) were incubated for 5 h with two-fold serial dilutions of UR-CG072 in the range of 0–8 nM and with (non-specific binding, open black circle) or without (total binding, filled blue circle) 3.7 µM scopolamine. The background-corrected cells' fluorescence intensities were determined by the cell detection algorithm as described in 'Material and methods', and are presented as individual replicates from a representative experiment of three independent experiments performed in duplicate. Four images from different fields of view were taken from a single well.