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. 2022 Jun 8;12(6):220019. doi: 10.1098/rsob.220019

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© 2022 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 9. — Association and dissociation of UR-CG072 (2 nM) to and from live CHO-K1-hM₄R cells. Dissociation was initiated with 5 µM scopolamine after 185 min (indicated with the arrow). A representative experiment of three independent experiments performed in duplicates is shown. Duplicate values are shown on the graph as separate points, to account for the measurement time difference between the replicates. Four images from different fields of view were taken from a single well. For analysis the GraphPad Prism model ‘association then dissociation’ was used.